Anion-Exchange Columns
Biocomma’s Plasmid preparation Columns are based on Biocomma’s anionexchange chromatography. The resins are composed of small particles with
a uniform particle, to provide high yields and reproducible performance.
Extracted plasmid is ready to use in many downstream applications, including
sequencing, library construction, transcription, transfection.
Details
Features● High yield: direct isopropanol precipitation can yield up to 10mg of plasmid DNA
● High purity: used in compatible reagent kits, it has an endotoxin content of less than 0.1 EU/mL, meeting the requirements of most transfection experiments
● Widely applicable: suitable for molecular biology experiments such as restriction digestion, PCR, transformation, sequencing, and transfection
Comma Pure® Anion-Exchange Column Performance AssessmentThis product has been independently tested multiple times, demonstrating exceptional reliability and stability. It delivers high-purity nucleic acids (free from RNA, protein, and salt residues), ultra-low endotoxin levels, high yield, and maintains a high flow rate, ensuring high-quality output for various downstream applications.
| Cat.# |
repeat no |
plasmid |
time(min) |
A260/A280 |
A260/A230 |
Yield(μg) |
| GS30-1 |
repeat 1 |
High Copy Plasmid:pUC 19 |
20.5 |
2.01 |
2.51 |
361.95 |
| repeat 2 |
32 |
2 |
2.52 |
327.1 |
| GS30-2 |
repeat 1 |
22.5 |
1.95 |
2.24 |
530.85 |
| repeat 2 |
25.1 |
1.94 |
2.3 |
525.35 |
| GS60-1 |
repeat 1 |
High Copy Plasmid:pUC 19 |
22.6 |
1.985 |
2.52 |
892 |
| repeat 2 |
25.1 |
1.995 |
2.51 |
898.9 |
| GS60-2 |
repeat 1 |
28.1 |
1.95 |
2.44 |
1210.9 |
| repeat 2 |
29.3 |
1.985 |
2.365 |
1252.55 |
Ordering Information
| Cat.# |
Description |
Volume |
Yield |
Qty |
| GS30-1 |
Plasmid Maxiprep Columns |
30 mL |
300-600 μg |
20pcs/pk |
| GS30-2 |
Plasmid Maxiprep Columns |
30 mL |
500-1000 μg |
20pcs/pk |
| GS60-1 |
Plasmid Maxiprep Columns |
60 mL |
1.0-1.5 mg |
10pcs/pk |
| GS60-2 |
Plasmid Maxiprep Columns |
60 mL |
1.0-3.0 mg |
10pcs/pk |
| GS300-22 |
Plasmid Megaprep Columns |
300 mL |
5-10 mg |
10pcs/pk |
| Customize |
| Note:Recommend 80-120 mL bacteria liquid for GS30 column. 100-300 mL bacteria liquid for GS60 column. 600-1000 mL bacteria liquid for GS300-22 column. |
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